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I want novel mutations to my protein!! Here I’m performing a quikchange expeirment. I’m introducing a DNA mutation into the DNA that encodes my protein of interest. I designed forward and reverse primers that have instructions to introduce my mutation at the correct location in my protein’s DNA sequence. I can check if my DNA mutation was successfully made by performing a bacteria transformation with this quickchange reaction material, plucking a few colonies, performing a miniprep experiment, a
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